#3293 ESTRADIOL REPLACEMENT MITIGATED BLOOD PRESSURE ELEVATION VIA SUPPRESSION OF NCC IN ANGIOTENSIN II-INFUSED OVARIECTOMIZED FEMALE RATS

Abstract Background and Aims Female rats excrete more urine sodium than male with similar blood pressure (BP). However, it is not clear whether the sex difference in natriuresis conserved menopausal rats. We assessed natriuretic response renal transporter activity ovariectomized female infused angiotensin II (ANGII) could be affected by estrogen supplementation. Method Six-week-old Sprague-Dawley (n=30) were (OVX, n =20) or sham-operated (n=10) (−4 weeks, −4W). After 2 subcutaneous estradiol was administered to half of OVX for 4 weeks (-2W∼2W). ANGII via osmotic minipump 60% (0W∼2W). Six groups (female saline: FS, ANGII: FA, OS, OA, OES, OEA) sacrificed after infusion (2W). also analyzed human data from Korean Genome Epidemiology Study (KoGES). Results ANGII-induced BP elevation highest OA group, followed FA OEA groups. Urine tended increase but decreased OA. Western blot results showed that cortical phosphorylated chloride cotransporter (pNCC) augmented treatment mitigated OEA. Cortical type receptor (AT2R) expression significantly higher estradiol-treated rats, irrespective infusion. Similarly, enhanced pNCC ANGII-treated distal convoluted tubular cells reversed treatment. In KoGES data, lower postmenopausal women premenopausal women, despite BP. Conclusion Estradiol ANGII-infused suppression AT2R.